serum fibroblast growth factor 21  (Proteintech)

Journal: GeroScience

Article Title: Kinetic proteomics identifies targeted changes in liver metabolism and the ribo-interactome by dietary sulfur amino acid restriction

doi: 10.1007/s11357-023-00758-w

Figure Lengend Snippet: Dietary sulfur amino acid restriction triggers hyperphagia, hyperdipsia, and attenuation of weight gain independent of dietary fat and energy content. A Study outline with information on biological sex, group names, mouse strain, dietary energy content, sulfur amino acid content, and key experimental interventions, including sample collection (small arrows) and intraperitoneal administration of deuterium (D 2 O inj.) and commencement of experimental diets and deuterium in drinking water (large arrow). The four diet groups are regular-fat control diet (RF.Ctrl, light grey square), regular-fat sulfur amino acid restricted diet (RF.SAAR, dark grey circle), high-fat control diet (HF.Ctrl, peach triangle), and high-fat sulfur amino acid restricted diet (HF.SAAR, red diamond). B Change in body weight of animals euthanized at the timepoints indicated. C Cumulative food intake normalized to body weight. D Cumulative water intake of animals euthanized at indicated timepoints. E Serum levels of fibroblast growth factor 21 (FGF21) measured in mice euthanized after 1, 7, or 14 days of consuming experimental diets. Data is displayed as mean ± standard error of the mean, with individual data points displayed as dots ( n = 3–5 male mice per group). Within each timepoint (indicated in grey headers), bars without shared letters were statistically different at α = 0.05, as determined by 3-way ANOVA followed by pair-wise t -tests with ( B , C , and D ) FDR or E Bonferroni’s correction for multiple comparisons

Article Snippet: Concentrations of serum FGF21 were estimated as previously described [ ] using a colorimetric sandwich ELISA (RD291108200R, BioVendor) according to the manufacturer’s instructions.

Techniques: Control

Journal: GeroScience

Article Title: Kinetic proteomics identifies targeted changes in liver metabolism and the ribo-interactome by dietary sulfur amino acid restriction

doi: 10.1007/s11357-023-00758-w

Figure Lengend Snippet: Seven days of dietary sulfur amino acid restriction activates the integrated stress response in the livers of male mice. A Hepatic ratios and representative blots of A phosphorylated (at S51) eIF2α over total eIF2α and B phosphorylated (at T389) p70 S6K over total p70 S6K in mice fed a regular-fat control (RF.Ctrl, light grey bars), RF sulfur amino acid restricted (RF.SAAR, dark grey bars), high-fat control (HF.Ctrl, peach bars), or HF.SAAR (red bars) for 7 days. C Hepatic ratios of (from left to right) Atf4 , Atf5 , Fgf21 , Gdf15 , Scd1 , and Nupr1 in mice fed indicated diets for 7 days. Data is displayed as mean ± standard error of the mean, with individual data points displayed as dots ( n = 3–5 mice per group). Bars without shared letters were statistically different at α = 0.05, as determined by 2-way ANOVA followed by pair-wise t -tests with Bonferroni’s correction for multiple comparisons

Article Snippet: Concentrations of serum FGF21 were estimated as previously described [ ] using a colorimetric sandwich ELISA (RD291108200R, BioVendor) according to the manufacturer’s instructions.

Techniques: Control